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Pullulanase

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Pullulanase
Debranching enzyme homotetramer, Klebsiella oxytoca
Identifiers
EC no.3.2.1.41
CAS no.9075-68-7
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Pullulanase (EC 3.2.1.41, limit dextrinase, amylopectin 6-glucanohydrolase, bacterial debranching enzyme, debranching enzyme, α-dextrin endo-1,6-α-glucosidase, R-enzyme, pullulan α-1,6-glucanohydrolase) is a specific kind of glucanase, an amylolytic exoenzyme, that degrades pullulan.[1][2][3] It is produced as an extracellular, cell surface-anchored lipoprotein by Gram-negative bacteria of the genus Klebsiella. Type I pullulanases specifically attack α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages. It is also produced by some other bacteria and archaea. Pullulanase is used as a processing aid in grain processing biotechnology (production of ethanol and sweeteners).

Pullulanase is also known as pullulan-6-glucanohydrolase (Debranching enzyme). Its substrate, pullulan, is regarded as a chain of maltotriose units linked by α-1,6-glycosidic bonds. Pullulanase will hydrolytically cleave pullulan (α-glucan polysaccharides).

Pullulanase enzyme in the food industry

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In the food industry, pullulanase works well as an ingredient. Pullulan can be applied directly to foods as a protective glaze or edible film due to its ability to form films. It can be used as a spice and flavoring agent for micro-encapsulation.[4] It is used in mayonnaise to maintain consistency and quality. It is additionally used in low-calorie food formulations as a starch replacement.

Pullulanase can be used to convert starches in grains into fermentable sugars, which yeast can use to produce alcohol during fermentation.

References

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  1. ^ Lee EY, Whelan WJ (1972). "Glycogen and starch debranching enzymes". In Boyer PD (ed.). The Enzymes. Vol. 5 (3rd ed.). New York: Academic Press. pp. 191–234.
  2. ^ Bender H, Wallenfels K (1966). "Pullulanase (An amylopectin and glycogen debranching enzyme) from Aerobacter aerogenes". Complex Carbohydrates. Methods in Enzymology. Vol. 8. pp. 555–559. doi:10.1016/0076-6879(66)08100-X. ISBN 9780121818081.
  3. ^ Manners DJ (1997). "Observations on the specificity and nomenclature of starch debranching enzymes". J. Appl. Glycosci. 44: 83–85.
  4. ^ Yatmaz E, Turhan (1995). "Pullulan production by fermentation and usage in the food industry". GIDA-Journal of Food. 2: 95–102.
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